HPLC Applications
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In the following you will find a list of our HPLC applications, which you can filter for compounds or phases via the search field. If you have questions concerning an application shown here or be searching for information on a separation not included in this list, please contact us under labor@cs-chromatographie.de!
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| Application Name | Substance | Synonyms | P/N | Phase | Dimension | Flow | Detector | Eluent | Temperature | Comment |
|---|---|---|---|---|---|---|---|---|---|---|
| 16 PAHs | PAHs | Polycyclic Aromatic Hydrocarbons | 58397012 | Multospher PAH III | 126 x 3 mm | 0,4 mL/min | UV 254 nm | Gradient; A = Water, B = Acetonitrile; 0 min - B = 55 %; 2 min - B = 55 %; 20 min - B = 100 %; 32 min - B = 100 % | RT | |
| 16 PAHs on MultoHigh 100 RP18 | PAHs | Polycyclic Aromatic Hydrocarbons | 586117215 | MultoHigh 100 RP 18-5µ | 150 x 4.6 mm | 1,0 mL/min | UV 254 nm | Gradient; refer to chromatogram | RT | |
| Aromatics on MultoHigh 100 RP18 | Propylparabene, Naphthalene, p-Xylene, Phenanthrene, Anthracene, Pyrene | 586117215 | MultoHigh 100 RP 18-5µ | 150 x 4.6 mm | 1,0 mL/min | UV 254 nm | Methanol : Water (80 : 20) | RT | ||
| Aromatic Amino Acids | p-Tyrosine, m-Tyrosine, α-Methyl-p-Tyrosine, o-Tyrosine, Phenylalanine | 584777 | Multospher 120 RP 18 AQ-5µ | 250 x 4 mm | 0,9 mL/min | FL 275/305, 58/288 | Gradient; A = 5 mM Ammonium formate (pH 4), B = Acetonitrile; 0 min - B = 0 %; 17 min - B = 0 %; 19 min - B = 70 %; 29 min - B = 70 % | 35 °C | Literature: J. Huňková, Dissertation, University of Hamburg (2003) | |
| Basic Drugs (Multospher AQ) | Uracil, Propanolol, Toluene, Doxepine, Amitriptyline | 58657715 | Multospher 120 RP 18 AQ-5µ | 150 x 4.6 mm | 1 mL/min | UV 220 nm | Methanol : 30 mM Phosphate Buffer (pH 6) (80 : 20) | RT | Red Chromatogram: Competitor Column AQ (150 x 4.6 mm) | |
| Sugars and Sugar Alcohols | Saccharose, Glucose, Galactose, Fructose, Mannitol, Sorbitol | 52898130 | Carbohydrate Ca²+ | 300 x 8 mm | 0,5 mL/min | RI | Water | 50 °C | ||
| Sugars and Sugar Alcohols | Maltotriose, Saccharose, Glucose, Galactose, Fructose, Mannitol, Sorbitol | 52898130 | Carbohydrate Ca²+ | 300 x 8 mm | 0,5 mL/min | RI | Water | 85 °C | ||
| Cortisones II | Prednisolone, Cortisone, Dexamethasone, Prednisolone-21-acetate, Hydrocortisone-21-acetate, Cortisone acetate | 584777 | Multospher 120 RP18 AQ-5µ | 250 x 4 mm | 1,0 mL/min | UV 254 nm | Acetonitrile : Water (1 : 1) + 0.1 % HCl (5 N) | RT | ||
| Cortisones I | Prednisolone, Cortisone, Dexamethasone, Prednisolone-21-acetate, Hydrocortisone-21-acetate, Cortisone acetate | 5841270 | Multospher 120 RP18 HP-5µ | 250 x 4 mm | 1,0 mL/min | UV 254 nm | Acetonitrile : Water (1 : 1) + 0.1 % HCl (5 N) | RT | ||
| Aromatics | Propylparabene, Naphthalene, p-Xylene, Phenanthrene, Anthracene, Pyrene | 586117215 | MultoHigh 100 RP 18-5µ | 150 x 4.6 mm | 1,0 mL/min | UV 254 nm | Methanol : Water (80 : 20) | RT | ||
| Engelhardt Test on Kromasil C18 | Uracil, Aniline, Phenol, 4-Ethylaniline, N,N-Dimethylaniline, Ethyl benzoate, Toluene, Ethylbenzene | 584919 | Kromasil 100-5 C18 | 250 x 4 mm | 1,0 mL/min | UV 254 nm | Methanol : Water (51 : 49) | RT | ||
| Engelhardt Test on Multospher FBS | Uracil, Aniline, Phenol, 4-Ethylaniline, N,N-Dimethylaniline, Ethyl benzoate, Toluene, Ethylbenzene | 584878 | Multospher 100 RP 18-5 FBS | 250 x 4 mm | 1,0 mL/min | UV 254 nm | Methanol : Water (51 : 49) | RT | ||
| Engelhardt Test on Multospher RP18-HP | Uracil, Aniline, Phenol, 4-Ethylaniline, N,N-Dimethylaniline, Ethyl benzoate, Toluene, Ethylbenzene | 5841271 | Multospher 120 RP18 HP-5µ | 250 x 4 mm | 1,0 mL/min | UV 254 nm | Methanol : Water (51 : 49) | RT | ||
| Engelhardt Test on ProntoSIL ace-EPS | Uracil, Aniline, Phenol, 4-Ethylaniline, N,N-Dimethylaniline, Ethyl benzoate, Toluene, Ethylbenzene | 58425PR05F29 | ProntoSIL 120-5-C18 ace-EPS | 250 x 4 mm | 1,0 mL/min | UV 254 nm | Methanol : Water (51 : 49) | RT | ||
| Gluconate Ketogluconate (Org. Acid) | Impurity, 2,5-Diketogluconate, 2-Ketogluconate, 5-Ketogluconate, Gluconate, Impurity | 52898030 | Organic Acid | 300 x 8 mm | 0,4 mL/min | UV 254 nm | 0.005 M Sulfuric Acid | 20 °C | ||
| Gluconate Ketogluconate (Org. Acid) | C16-Sphinganine, 4-Hydroxysphinganine, E-Sphing-4-enine, C20-Sphinganine, C20-(E)-4-Sphinganine | 584875 | Multospher 100 RP 18-5µ | 250 x 4 mm | 0,8 mL/min | UV 350 nm | Gradient; A = Methanol : Acetonitrile : 2-Propanol (10 : 3 : 1), B = Water; 0 min - B = 16 %; 55 min - B = 0 % (concave) | RT | Literature: P. Ternes, S. Franke, U. Zähringer, P. Sperling, E. Heinz (2002), J. Biol. Chem. 277, 25512-25518 | |
| 6 Cations | Sodium, Potassium, Magnesium, Calcium, Strontium, Barium | 546976 | Multospher Kation | 125 x 4.6 mm | 1,50 mL/min | Conductivity | 0,61 mM Ascorbic Acid + 1,96 mM Oxalic Acid | 45 °C | ||
| Caffeine in Beverages | Coffee, Energy Drink, Decaffeinated Coffee | 5831228-H | Multospher APS-HP 3µ HILIC | 250 x 3 mm | 0,6 mL/min | UV 254 nm | Acetonitrile : Ammonium Acetate Solution 5 mM (95 : 5) | RT | ||
| Caffeine in Beverages | Mannitol, Lacrulose | 5831228-H | Multospher APS-HP 3µ HILIC | 250 x 3 mm | 0,8 mL/min | RI | Acetonitrile : Water (70 : 30) | 20 °C | ||
| Oligonucleotides | CAAGACGCAA, CAACCAACGT, GGTGATCAAC, CCCTGAACAA, CGTGTATTGG, GGTCCTATAC | Oligonucleotide | 58657415 | Multospher 120 RP18-AQ-5µ | 150 x 4.6 mm | 1 mL/min | UV 260 nm | Gradient; A = Potassium Phosphate Solution 50 mM (pH 7), B = Acetonitrile; 0 min - B = 5 %; 20 min - B = 15 % | RT | |
| Organic Acids + Sugars | Lactose, Citrate, Glucose, Galactose, Succinate, Lactate, Formate, Acetate, Propionate | Citric Acid, Succinic Acid, Lactic Acid, Formic Acid, Acetic Acid, Propionic Acid, Propanoic Acid | 52898030 | Organic Acid | 300 x 8 mm | 0,4 mL/min | RI | 0,01 N Sulfuric Acid | 60 °C | |
| Organic Acids + Sugars | Saccharose, Glucose, Fructose, Lactate, Formate, Acetate, Propionate | Sucrose, Lactic Acid, Acetic Acid, Propionic Acid, Propanoic Acid | 52898030 | Organic Acid | 300 x 8 mm | 0,8 mL/min | RI | 0,008 N Sulfuric Acid | 20 °C | |
| Organic Acids | Ascorbic acid, Lactic Acid, Acetic Acid, Propionic Acid | Vitamin C, Lactate, Acetate, Propionate, Propanoate, Propanoic Acid | 52898030 | Organic Acid | 300 x 8 mm | 0,8 mL/min | UV 220 nm | 0,008 N Sulfuric Acid | 20 °C | |
| Organic Acids | Citric acid, Tartaric acid, Malic acid, Lactic acid, Acetic acid, Fumaric acid | Citrate, Tartrate, Lactate, Acetate, Fumarate | 52898030 | Organic Acid | 300 x 8 mm | 0,6 mL/min | UV 210 nm | refer to chromatogram | refer to chromatogram | |
| Phenols | para-Hydroxybenzaldehyde, Vanillic acid, Syringic acid, Vanilline, Syringa aldehyde, Acetonvanillone, Acetonsyringone | 683919 | MultoKrom 100-5 C18 | 250 x 3 mm | 0,8 mL/min | UV 254 nm | Gradient; A = Water : Methanol (95 : 5), B = Water : Methanol (5 : 95); 0 min - B = 0 %; 3 min - B = 10 %; 12 min - B 55 %; 15 min - B 55 %; 30 min - B 60 % | 60 °C | ||
| Phthalates | Bis-2-ethylhexyl phthalate, Diethyl phthalate, Diphenyl phthalate | 5841235 | MultoHigh 100-5 Diol | 250 x 4 mm | 1,3 mL/min | UV 254 nm | Hexane : Dichloromethane : Methanol (95 : 4 : 1) | RT | ||
| Silanol Activity (Acid-Base Test) | Pyridine, Phenol | 5861172 | MultoHigh 100 RP 18-5 | 125 x 4.6 mm | 1,3 mL/min | UV 254 nm | Acetonitrile : Water (40 : 60) | RT | ||
| Sulfonamides | Sulfanilamide, Sulfamethazine, Sulfamerazine | 5841271 | Multospher 120 RP 18 HP-5µ | 250 x 4 mm | 1 mL/min | UV 254 nm | Acetonitrile : Potassium Dihydrogen Phosphate Solution (pH 3) (20 : 80) | RT | ||
| Thiourea | Toluene, Thiourea | 5831273-H | MultoHigh 100 Si 5µ HILIC | 250 x 3 mm | 0,5 mL/min | UV 254 nm | Acetonitrile : Water (80 : 20) | RT | ||
| Tricyclic Antidepressants | Uracil, Desipramine, Nortriptyline, Toluene, Doxepine, Amitriptyline | 586117215 | MultoHigh 100 RP18-5µ | 150 x 4.6 mm | 1 mL/min | UV 220 nm | Methanol : Phosphate Buffer 30 mM (pH 6) (80 : 20) | RT | ||
| Uracil, 5-Fluorouracil | 5-Fluorouracil, Uracil | 5841233-H | MultoHigh 100 CN-5µ | 250 x 4 mm | 1 mL/min | UV 254 nm | Acetonitrile : Water (80 : 20) | RT | ||
| Uracil, 5-Fluorouracil | Uracil, 5-Fluorouracil | 5831228-H | Multospher APS-HP-3µ HILIC | 250 x 3 mm | 1,5 mL/min | UV 254 nm | Acetonitrile : Ammonium Acetate Buffer 5 mM (95 : 5) | RT | ||
| Trehalose, Maltose, Glucose | Trehalose, Maltose, Glucose | 52898230 | Carbohydrate Pb²+ | 300 x 8 mm | 0,4 mL/min | RI | Water | 60 °C | ||
| Sugars | Fructose, Glucose, Galactose, Sucrose, Maltose, Lactose | 5831228 | Multospher APS-HP-3µ HILIC | 250 x 3 mm | 0,8 mL/min | RI | Acetonitrile : Water (80 : 20) | RT | ||
| Sugars + Sorbitol | Fructose, Glucose, Sorbitol, Sucrose, Maltose, Lactose | 583190710 | MultoHigh U-NH2 HILIC | 100 x 3 mm | 1,5 mL/min | RI | Acetonitrile : Water (80 : 20) | RT | ||
| Sugars | Ribose, Xylose, Arabinose, Fructose, Glucose | 5841128-H | Multospher APS-HP-3µ HILIC | 125 x 4 mm | 1 mL/min | RI | Acetonitrile : Water (80 : 20) | RT | ||
| Sugars | Fructose, Glucose, Sucrose, Maltose, Lactose | 583190310 | MultoHigh U APS | 100 x 3 mm | 0,8 mL/min | RI | Acetonitrile : Water (80 : 20) | RT | ||
| Sugars + Sugar Alcohols | Xylitol, Fructose, Sorbitol, Mannitol, Glucose, Sucrose, Maltitol, Maltose, Lactose, Lactitol | 583190715 | MultoHigh U-NH2 HILIC | 150 x 3 mm | 1,5 mL/min | RI | Acetonitrile : Water (85 : 15) | RT | ||
| Sugars + Sugar Alcohols | Sucrose, Glucose, Fructose, Glycerol, Ethanol | Glycerin | 52898030 | Organic Acid Resin | 300 x 8 mm | 0,6 mL/min | RI | 9 mM Sulfuric Acid | RT | |
| Cytosolic Nucleotides and Related Compounds | cyclic ADP-ribose, NAD, Nicotinic Acid, NAAD, cAMP, ADP, ATP, 2'-P-ADPR, Nicotinamide, AMP, cGMP, ADPR, NADP, GTP, NAADP | 684831 | Multohyp BDS-C18-5µ | 250 x 4 mm | 1 mL/min | UV 270 nm | Gradient; A = 20 mM Potassium dihydrogen phosphate solution + 5 mM TBAHP, pH 6, B = Methanol; 0 min - B = 15 %; 3,5 min - B = 15 %; 5,5 min - B = 32,5 %; 9 min - B = 40 %; 11 min - B = 50 %; 16 min - B = 50 %; 18 min - B = 15 %; 27 min - B = 15 % | RT | Literature: A. Gasser, A. H. Guse, J. Chromatogr. B 821 (2005) 181-187 | |
| Insulin | Insulin (bovine), Insulin (human), Insulin (porcine) | 586117615 | MultoHigh Bio 200-5-C8 | 150 x 4.6 mm | 1 mL/min | UV 215 nm | Gradient; A = Water + 0,1 % TFA, B = Acetonitrile + 0,1 % TFA; 0 min - B = 29 %; 30 min - B = 32 % | 30 °C | ||
| Peptides | Arg8-Vassopressin, Bradykinin (Seq. 1-5), Oxytocin, Met-Enkephalin, LHRH, Leu-Enkephalin, Bradykinin, Bombesin, Impurity | 586117615 | MultoHigh Bio 200-5-C8 | 150 x 4.6 mm | 1 mL/min | UV 220 nm | Gradient; A = Water + 0,1 % TFA, B = Acetonitrile; 0 min - B = 20 %; 60 min - B = 80 % | 30 °C | ||
| Standard Proteins | Ribonuclease A (MW 13700), Cytochrome C (MW 12400), Lysozyme (MW 14300), BSA (MW 67000), Myoglobin (MW 18800), Ovalbumin (MW 45300) | 586117715 | MultoHigh Bio 300-5-C4 | 150 x 4.6 mm | 1 mL/min | UV 220 nm | Gradient; A = Water + 0,1 % TFA, B = Acetonitrile + 0,1 % TFA; 0 min - B = 20 %; 25 min - B = 60 % | 37 °C | ||
| Tryptic Digest BSA | Tryptic Digest BSA | 586117615 | MultoHigh Bio 200-5-C8 | 150 x 4.6 mm | 1 mL/min | UV 220 nm | Gradient; A = Water + 0,1 % TFA, B = Acetonitrile; 0 min - B = 10 %; 60 min - B = 60 % | 30 °C | ||
| Pancreatic Digest of Casein Peptone (200C18) | Pancreatic Digest | 586117615 | MultoHigh Bio 200-5-C8 | 150 x 4.6 mm | 1 mL/min | UV 220 nm | Gradient; A = Water + 0,1 % TFA, B = Acetonitrile : Water (70 : 30) + 0,1 % TFA; 0 min - B = 5 %; 40 min - B = 41 % | RT | ||
| Pancreatic Digest of Casein Peptone (300C4) | Pancreatic Digest | 586117715 | MultoHigh Bio 300-5-C4 | 150 x 4.6 mm | 1 mL/min | UV 220 nm | Gradient; A = Water + 0,1 % TFA, B = Acetonitrile : Water (70 : 30) + 0,1 % TFA; 0 min - B = 5 %; 40 min - B = 41 % | RT | ||
| Pancreatic Digest of Casein Peptone (aceEPS) | Pancreatic Digest | 58612PR05F2915 | ProntoSIL C18 ace-EPS | 150 x 4.6 mm | 1 mL/min | UV 220 nm | Gradient; A = Water + 0,1 % TFA, B = Acetonitrile : Water (70 : 30) + 0,1 % TFA; 0 min - B = 5 %; 40 min - B = 41 % | RT | ||
| Tryptic Digest Caseine | Tryptic Digest Caseine | 586117615 | MultoHigh Bio 200-5-C8 | 150 x 4.6 mm | 1 mL/min | UV 220 nm | Gradient; A = Wasser + 0,1 % TFA, B = Acetonitril; 0 min - B = 10 %; 60 min - B = 60 % | 30 °C | ||
| Antiepileptics | Primidone, Phenytoin, Carbamazepine | 586117215 | MultoHigh 100 RP18-5 | 150 x 4.6 mm | 1 mL/min | UV 220 nm | 20 mM Phosphate Buffer (pH 7) : Acetonitrile (30 : 70) | RT | ||
| Antiphlogistics | Piroxicane, Sulindac, Ketoprofene, Naproxene, Flurbiprofene, Ibuprofene | 586117215 | MultoHigh 100 RP18-5 | 150 x 4.6 mm | 1 mL/min | UV 260 nm | Acetonitrile : 1 % aqueous Acetic Acid (65 : 35) | 40 °C | ||
| β Blockers | Atenolol, Nadolol, Metoprolol, Propanolol, Alprenolol, Alprenolol | 586117215 | MultoHigh 100 RP18-5 | 150 x 4.6 mm | 1 mL/min | UV 220 nm | 20 mM Phosphate Buffer (pH 7) : Methanol (40 : 60) | 40 °C | ||
| Catecholamines | Norepinephrin, Epinephrin, Dopamine, DOPA | Noradrenalin, Adrenalin | 586117215 | MultoHigh 100 RP18-5 | 150 x 4.6 mm | 1 mL/min | UV 280 nm | Methanol : 20 mM Sodium Dihydrogen Phosphate Solution (1 : 99) | RT | |
| Cinchona Alkaloids | Cinchonine, Cinchonidine, Quinidine, Quinine | 586117215 | MultoHigh 100 RP18-5 | 150 x 4.6 mm | 1 mL/min | UV 235 nm | Methanol : 20 mM Sodium Dihydrogen Phosphate Solution (35 : 65) | 40 °C | ||
| Congestion Medicines (APIs) | Acetaminophen, Caffeine, Chlorpheniramine maleate, Aspirin, Ethenzamide, Bucetine | Acetylsalicylic Acid | 586117215 | Multohigh 100 RP18-5 | 150 x 4.6 mm | 1 mL/min | UV 235 nm | 50 mM Phosphate Buffer (pH 2.5) : Methanol (80 : 20) | 40 °C | |
| Flavonoids (1) | Quercetine, Kaempferol | 586117215 | MultoHigh 100 RP18-5 | 150 x 4.6 mm | 1 mL/min | UV 260 nm | 10 mM Phosphoric Acid : Acetonitrile (65 : 35) | 40 °C | ||
| Flavonoids (2) | Rutine, Quercitrine, Quercetine, Kaempferol | 586117215 | MultoHigh 100 RP18-5 | 150 x 4.6 mm | 1 mL/min | UV 260 nm | 0.1 % Phosphoric Acid : Methanol (50 : 50) | 40 °C | ||
| Histamine Antagonists | Pheniramine, Doxylamine, Chlorpheniramine, Diphenylhydramine | 586117215 | MultoHigh 100 RP18-5 | 150 x 4.6 mm | 1 mL/min | UV 254 nm | 20 mM Phosphate Buffer (pH 7) : Methanol (40 : 60) | 40 °C | ||
| Nitrite/Nitrate Determination | Nitrite, Nitrate | 5841128 | Multospher APS-HP-5µ | 125 x 4 mm | 0,8 mL/min | UV 210 nm | 20 mM Phosphate Buffer (pH 2.8) | 40 °C | ||
| Nucleoside | Cytosine, Uracil, Cytidine, Uridine, Thymine | 5861272 | MultoHigh 100 RP18-5 | 250 x 4.6 mm | 1 mL/min | UV 254 nm | Water | 40 °C | ||
| Organic Acids (1) | Lactic acid, Acetic acid, Citric acid, Succinic acid, Propionic acid | Lactate, Acetate, Citrate, Succinate, Propionate, Propanoic Acid, Propanoate | 586117215 | MultoHigh 100 RP18-5 | 150 x 4.6 mm | 1 mL/min | UV 220 nm | 0.1 % Phosphoric Acid : Acetonitrile (98 : 2) | RT | |
| Organic Acids (2) | Formic acid, Lactic acid, Acetic acid, Citric acid, Succinic acid, Propionic acid | Formate, Lactate, Acetate, Citrate, Succinate, Propionate, Propanoic Acid, Propanoate | 586117215 | MultoHigh 100 RP18-5 | 150 x 4.6 mm | 1 mL/min | UV 220 nm | 0.1 % Phosphoric Acid : Acetonitrile (98 : 2) | RT | |
| Penicillin | Amoxicillin, Ampicillin | 586117215 | MultoHigh 100 RP18-5 | 150 x 4.6 mm | 1 mL/min | UV 220 nm | 20 mM Potassium Dihydrogen Phosphate and 5 mM Sodium 1-Decanesulfonate in Acetonitrile | 40 °C | ||
| Procainamide | Uracil, Procainamide, N-Acetylprocainamide | 586117215 | MultoHigh 100 RP18-5 | 150 x 4.6 mm | 1 mL/min | UV 254 nm | 20 mM Phosphate Buffer (pH 7) : Methanol (40 : 60) | 40 °C | ||
| Saccharin in Galvanic Baths | Saccharin | 5861272 | MultoHigh 100 RP18-5 | 250 x 4.6 mm | 1 mL/min | UV 200 nm | Water : Methanol (50 : 50) + 0.3 % Phosphoric Acid | RT | ||
| Steroids | Estriol, β-Estradiol, Estrone, Progesterone | 586117215 | MultoHigh 100 RP18-5 | 150 x 4.6 mm | 1 mL/min | UV 230 nm | Water : Acetonitrile (40 : 60) | RT | ||
| Sulfonamides | Sulfanilamide, Sulfisomidine, Sulfadiazine, Sulfamethazine, Sulfamonomethoxine | 586117215 | MultoHigh 100 RP18-5 | 150 x 4.6 mm | 1 mL/min | UV 254 nm | 10 mM Phosphoric Acid : Acetonitrile (85 : 15) | 40 °C | ||
| Tricyclic Antidepressants | Protriptyline, Desipramine, Nortriptyline, Doxepine, Imipramine, Amitriptyline, Trimipramine | 586117215 | MultoHigh 100 RP18-5 | 150 x 4.6 mm | 1.2 mL/min | UV 240 nm | 20 mM Phosphate Buffer (pH 7) : Methanol (30 : 70) | RT | ||
| Vitamins (water soluble) 2 | Vitamin C (L-Ascorbic Acid), Vitamin B3 (Nicotinic Acid), Vitamin B1 (Thiamine), Vitamin B3 (Nicotinamide), Vitamin B5 (Pantothenic Acid), Vitamin B9 (Folic Acid), Vitamin B7 (Biotine) , Vitamin B12 (Cyanocobalamine), Vitamin B2 (Riboflavine) | Ascorbate, Nicotinate, Pantothenate, Folate | 586117215 | MultoHigh 100 RP18-5 | 150 x 4.6 mm | 1 mL/min | UV 210 nm | Gradient; A = 20 mM Ammonium Dihydrogen Phosphate Solution, B = Acetonitrile; 0 min - B = 2 %; 20 min - B = 22 % | 40 °C | |
| Vitamins (water soluble) | Vitamin C (L-Ascorbic Acid), Vitamin B13 (Orotic Acid), Vitamin B3 (Nicotinic Acid), Nicotinamide, Pyridoxal, Vitamin B6 (Pyridoxine), Vitamin B2 , Vitamin B1 (Thiamine) | Ascorbate, Orotate, Nicotinate, Nicotinic Acid Amide | 5861272 | MultoHigh 100 RP18-5 | 250 x 4.6 mm | 1 mL/min | UV 210 nm | 20 mM Phosphoric Acid + 5 mM Sodium 1-Pentanesulfonate : Acetonitrile (92 : 8) | 40 °C | |
| Sugars (1) | Fructose, Glucose, Sucrose, Maltose | 5841229-H | Multospher 120-APS-HP-5µ | 250 x 4 mm | 1.5 mL/min | RI | Acetonitrile : Water (75 : 25) | RT | ||
| Stevia Glycosides | Stevioside, Rebaudioside C, Rebaudioside A | 5831907-05-H / 5831229-H | MultoHigh U-NH2 HILIC / Multospher-APS-HP 5µ HILIC | refer to chromatogram | 1 mL/min | UV 210 nm | Acetonitrile : Water (80 : 20) | RT | ||
| Sugars on Carbohydrate Pb2+ | Melecitose, Sucrose, Lactose, Glucose, Lactulose, Galactose, Fucose, Fructose | 52898230 | Carbohydrate Pb2+ | 300 x 8 mm | 0,4 mL/min | RI | Water | 85 °C | ||
| Sugars on Carbohydrate Pb2+ | Raffinose, Maltose, Glucose, Xylose, Galactose, Arabinose, Mannose | 52898230 | Carbohydrate Pb2+ | 300 x 8 mm | 0,4 mL/min | RI | Water | 85 °C | ||
| Sugars on Carbohydrate Pb2+ | Sucrose, Glucose, Galactose, Fructose | 52898230 | Carbohydrate Pb2+ | 300 x 8 mm | 0,8 mL/min | RI | Water | 80 °C | ||
| Aprotic-Polar Solvents | Acetonitrile, Dimethyl sulfoxide, Tetrahydrofuran, Dimethyl formamide | ACN, DMSO, THF, DMF | 52898030 | Organic Acid | 300 x 8 mm | 2,0 mL/min | RI | 4 mM Sulfuric Acid | 75 °C | |
| E-Cigarette Liquid on Organic Acid | Glycerol, Ethylene glycol, 1,2-Propanediol | Glycerol, Glycol, Propylene Glycol | 52898030 | Organic Acid | 300 x 8 mm | 0,8 mL/min | RI | 0,01 M Sulfuric Acid | 70 °C | |
| Sugars on MultoHigh SO3H-5µ | Xylose, Fructose, Glucose, Sorbitol, Sucrose, Maltose, Trehalose, Maltotriose | 584 XXX | MultoHigh SO3H-5µ | 250 x 4 mm | 1,3 mL/min | RI | Acetonitrile : Water (80 : 20) | RT | ||
| Aromatics on CS-ODS-5µ | Naphthalene, p-Xylene, Phenanthrene, Anthracene, Pyrene | 586 1310 15 | CS-ODS 100-5µ | 150 x 4.6 mm | 1,3 mL/min | UV 254 nm | Acetonitrile : Water (80 : 20) | RT | ||
| Nucleosides on CS-ODS-5µ | Cytosine, Uracil, Cytidine, Uridine | 586 1310 15 | CS-ODS 100-5µ | 150 x 4.6 mm | 1,3 mL/min | UV 254 nm | Water | RT | ||
| Sugars on MultoKrom PEI-5µ | Xylose, Fructose, Glucose, Sucrose, Lactose | 584XXX | MultoKrom PEI-5µ | 250 x 4 mm | 2,0 mL/min | RI | Acetonitrile : Water (80 : 20) | RT | ||
| Sugar alcohols on MultoKrom PEI-5µ | Xylitol, Sorbitol, Mannitol | 584XXX | MultoKrom PEI-5µ | 250 x 4 mm | 1,3 mL/min | RI | Acetonitrile : Water (80 : 20) | RT | ||
| Sugars and sugar alcohols on MultoKrom PEI-5µ | Xylose, Xylitol, Fructose, Sorbitol, Mannitol, Glucose, Sucrose, Lactose | 584XXX | MultoKrom PEI-5µ | 250 x 4 mm | 2,0 mL/min | RI | Acetonitrile : Water (80 : 20) | RT | ||
| Sugars and sugar alcohols on Carbohydrate Ca2+ | Maltotriose, Lactose, Glucose, Xylose, Fructose, Arabitol, Xylitol | 52898130 | Carbohydrate Ca2+ | 300 x 8 mm | 0,5 mL/min | RI | Water | 85 °C | ||
| Sugars and sugar alcohols on Carbohydrate Pb2+ | Sucrose, Glucose, Xylose, Galactose, Arabinose, Fructose, Mannitol, Sorbitol | 52898230 | Carbohydrate Pb2+ | 300 x 8 mm | 0,5 mL/min | RI | Water | 85 °C | ||
| Sugars and sugar alcohols on Carbohydrate Pb2+ | Maltotriose, Lactose, Lactulose, Galactose, Arabinose, Fructose, Adonitol, Xylitol | 52898230 | Carbohydrate Pb2+ | 300 x 8 mm | 0,5 mL/min | RI | Water | 85 °C | ||
| Nitrogen Heterocycles on MultoKrom PEI-5µ | Caffeine, Cytosine, Uridine, Guanosine | 584XXX | MultoKrom PEI-5µ | 250 x 4 mm | 1,3 mL/min | UV 254 nm | Water | RT | ||
| PFAS Standard (20 PFAS) | PFBA, PFPeA, PFHxA, PFBS, PFHpA, PFPeS, PFOA, PFHxS, PFNA, PFHpS, PFDA, PFOS, PFUnDA, PFNS, PFDoDA, PFDS, PFTrDA, PFUnDS, PFDoDS, PFTrDS | 583157415 | Multospher PFAS | 150 x 3 mm | 0,5 mL/min | ESI(-)-MS | Gradient; A = 20 mM Triethanolamine in Water, adjusted to pH 8 with Acetic Acid, B = 20 mM Triethanolamine in Acetonitrile, same amount of Acetic Acid added as in A; 0 min - B = 10 %; 1 min - B = 40 %; 6,5 min - B = 90 %; 9 min - B = 90 %; 10 min - B = 10 % | RT | Instrument: Bruker SolariX FT-ICR-MS with API Source; ESI Capillary Voltage: 3500 V; m/z Range: 54 - 800; Avg. Scans: 1; Accumulation Time: 0,1 s; Method Development by Kevin Staudt, Servicestelle Massenspektrometrie der Universität des Saarlandes | |
| homologous acids on organic acid | Formic acid, Acetic acid, Propionic acid, Isobutyric acid, Butyric acid, Valeric acid, Capronic acid | Formate, Acetate, Propanoic acid, Propionate, Propanoate, Isobutyrate, iso-Butyrate, iso-Butanoate, Butyrate, Butanoate, Valerate | 52898030 | Organic Acid | 300 x 8 mm | 1,0 mL/min | UV 210 nm | Water | RT | |
| Organic acids on Organic Acid | Oxalic acid, Maleic acid, Tartaric acid, Malic acid, Succinic acid, Formic acid, Acetic acid | Oxalate, Tartrate, Succinate, Formate, Acetate | 52898030 | Organic Acid | 300 x 8 mm | 0,8 mL/min | UV 210 nm | Water | RT | |
| Organic acids on Organic Acid | Oxalic acid, Citric acid, Tartaric acid, Ascorbic acid, Succinic acid, Formic acid, Fumaric acid, Acrylic acid | Oxalate, Citrate, Tartrate, Ascorbate, Vitamin C, Succinate, Formate, Fumarate, Acrylate | 52898030 | Organic Acid | 300 x 8 mm | 0,8 mL/min | UV 210 nm | Water | RT | |
| Ayran on Organic Acid | Oxalic acid, Citric acid, Ascorbic acid, Lactic acid, Formic acid, Acetic acid, Butyric acid | Oxalate, Citrate, Ascorbate, Vitamin C, Lactate, Formate, Acetate, Butyrate, Butanoate | 52898030 | Organic Acid | 300 x 8 mm | 0,8 mL/min | UV 210 nm | Water | RT | |
| Nitro Compounds on CS-ODS 100-5µ | 2,4-Dinitrophenol, 1-Nitropropane, 3-Nitroaniline, Picric acid, 2-Nitrophenol, 2-Nitroanisole, Nitrobenzene | 2,4,6-Trinitrophenol | 5862310 | CS-ODS 100-5µ | 250 x 4.6 mm | 1,5 mL/min | UV 230 nm | Methanol : 20 mM Sodium Dihydrogen Phosphate Solution (50 : 50) | RT | |
| Aromatics on CS ODS 100-5µ with rum as eluent | Phenol, m-Cresol, 2,4-Dimethylphenol, Benzene, Toluene, Xylene (mixture of isomers), Naphthalene, Biphenyl, Anthracene | 5841310 | CS-ODS 100-5µ | 125 x 4 mm | 1,0 mL/min | UV 254 nm | White Overproof Rum Wray & Nephew 63 % vol. | RT | ||
| Alcohols on Organic Acid | Glycerol, Ethylene glycol, 1,2-Propanediol, 1,3-Propanediol, Hydroxyacetone, Methanol, Ethanol, 2-Propanol, 1-Propanol | Glycerin, Glycol, Propylene glycol, Isopropanol, iso-Propanol | 52898030 | Organic Acid | 300 x 8 mm | 0,8 mL/min | RI | 0,008 N Sulfuric Acid | RT | |
| Polyring Systems on MultoKrom Phenyl | Bisphenol A, Benzene, Naphthalene, Biphenyl, Anthracene, Chrysene | BPA | 584XXX | MultoKrom 100-5 Phenyl | 125 x 4 mm | 1,0 mL/min | UV 254 nm | Acetonitrile:Water 80:20 | RT |