Details for HPLC Application No. 55

Cytosolic Nucleotides and Related Compounds

Column:

250 x 4 mm Multohyp BDS-C18-5µ

P/N:

684831

HPLC Conditions:

Eluent:	Gradient; A = 20 mM Potassium dihydrogen phosphate solution + 5 mM TBAHP, pH 6, B = Methanol; 0 min - B = 15 %; 3,5 min - B = 15 %; 5,5 min - B = 32,5 %; 9 min - B = 40 %; 11 min - B = 50 %; 16 min - B = 50 %; 18 min - B = 15 %; 27 min - B = 15 %
Flow:	1 mL/min
Temperature:	RT
Detector:	UV 270 nm

Peaks:

cyclic ADP-ribose
NAD
Nicotinic Acid
NAAD
cAMP
ADP
ATP
2'-P-ADPR
Nicotinamide
AMP
cGMP
ADPR
NADP
GTP
NAADP

Comment: Literature: A. Gasser, A. H. Guse, J. Chromatogr. B 821 (2005) 181-187